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KMID : 1007520160250010205
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Food Science and Biotechnology
2016 Volume.25 No. 1 p.205 ~ p.211
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Prickly Palm Cactus Husk as a Raw Material for Production of Ligninolytic Enzymes by Aspergillus niger
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Tamires Carvalho dos Santos
Nadabe dos Santos Reis
Fabiano de Paula Pereira Machado
Renata Cristina Ferereira Bonomo
Marcelo Franco
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Abstract
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Prickly palm cactus husk was used as a solid-state fermentation support-substrate for production of the ligninolytic enzymes laccase, peroxide manganese, and lignin peroxidase by Aspergillus niger. Effects of water activity, temperature, and fermentation time on enzymatic production were evaluated using a central composite rotatable design. Response surface methodology revealed that maximum enzyme production was achieved at 73.38 h of fermentation, a water activity of 0.87 Aw, at 28.74°C for laccase, at 65.33 h, 0.89 Aw, and 28.96°C for lignin peroxidase, and at 70.44 h, 0.91 Aw, and 28.84°C for manganese peroxidase. Optimized enzyme production was 9,023.67 UI/L for laccase, 2,234.75 UI/L for lignin peroxidase, and 8,534.81 UI/L for manganese peroxidase. Thermostability and pH stability were observed for all enzymes. Enzymatic deactivation kinetic experiments indicated that enzymes remained active after freezing of crude extracts.
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KEYWORD
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fermentation, biotransformation, statistical, semi-arid, optimisation
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